AN EVALUATION OF HAEMOGLOBIN DETERMINATION USING SODIUM LAURYL SULFATE

J. Kudher, Mohammad; J. Musa, Raad; Sh. Mansoor, Saad

	AN EVALUATION OF HAEMOGLOBIN DETERMINATION USING SODIUM LAURYL SULFATE
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 4, Issue 2, 2005, Pages 187-195
Authors
Mohammad J. Kudher; Raad J. Musa; Saad Sh. Mansoor
Abstract
Introduction: Various methods for the quantitative determination of Hb have been developed during the last decade, based on chemical and physical principles of Hb, gasometry or spectrophotometry. Methods: Spectrophotometric measurement of Hb as HiCN was carried out by the ICSH recommended reference method and by cyanide free (sodium lauryl sulfate) reagent. Measurements were carried out in 347 venous blood collected into K2EDTA anticoagulant. Normal samples were obtained from volunteers and other samples were obtained from patient specimens in routine diagnostic service. The latter included Leukaemia with high WBC count, Lipaemia, Cord blood, other abnormalities (uraemia, jaundice thalassemia minor and major) and methaemoglobinemia. The Hb value then determined by using HiCN method and SLS method. Results: Maximum absorbance is at 535 nm, the conversion time of Hb to SLS-Hb was rapid of less than 15 seconds. The freshly prepared SLS-Hb was stabile in the first 120 minutes after dilution. Haemoglobin converts almost instantaneously and there is a direct relationship of absorbance to Hb concentration over awid range of measurements (r = 0.999). Conclusion: its reliability is equal to that of HiCN method with routine blood specimens, but slightly more reliable than HiCN method when there is interference by lipaemia. There is no significant difference in measurements on samples containing HbF (r = 0.995). It measures methamoglobin with correlation coefficient (r) of 0.999. It has a major advantage in that the reagent is non-hazardous compound. Keywords: Haemoglobin, Sodium lauryl sulphate
Keywords
Haemoglobin; Sodium lauryl sulphate

Statistics Article View: 14