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THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE | ||
| Mesopotamia Journal of Agriculture | ||
| Article 26, Volume 39, Issue 4, 2011, Pages 237-243 PDF (0 K) | ||
| DOI: 10.33899/magrj.2011.28133 | ||
| Author | ||
| KHALID H.TAHA | ||
| Abstract | ||
| The result showed that optimum temperature for maximum activity of Chitinase was 25 Cº with 2.74 unit/µm/min. while optimum pH for maximum activity Chitinase was 5 with 2.25 unit/µm/min . Significant activities of chitinase was produced by T. harzianum in culture media amended with fresh mycelium of M. phaseolina , where the releasing of reducing sugar N-acetylglucosamin was 280 µg/ml which Significantly differ from T. harzianum culture media amended with fresh and dry mycelium of R.solani and F.solani where the releasing of reducing sugar N-acetylglucosamin was 200 µg/ml. Production of chitinase using a medium containing chitin reached 40 µg/ml . Maximium chitinase inhibition activity, purified by ammonium sulphate, was recorded for M. phaseolina mycelium growth 47.03% , which Significantly differ with of R.solani and F.solani where the inhibition activity was 35.18 and 33.33% respectively | ||
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