SUBJECTIVE AND QUANTITATIVE EVALUATION OF BONE MARROW TREPHINE BIOPSY IN IDIOPATHIC MYELOFIBROSIS

Al-Mashhadani, Ahlam; Sh. Mansoor, Saad; S. Sarhan, Falih

	SUBJECTIVE AND QUANTITATIVE EVALUATION OF BONE MARROW TREPHINE BIOPSY IN IDIOPATHIC MYELOFIBROSIS
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 4, Issue 2, 2005, Pages 214-219
Authors
Ahlam Al-Mashhadani; Saad Sh. Mansoor; Falih S. Sarhan
Abstract
Background: Idiopathic Myelofibrosis (IMF) is one of the myeloproliferative disorders in which various degrees of bone marrow fibrosis constitute the cardinal pathogenetic criteria for the disease therefore the study of bone marrow trephine biopsy is a major step in diagnosis . Objectives: Are reticulin and iron stain are essential for the diagnosis and staging of idiopathic myelofibrosis Methods: Trephine biopsies of 30 patients with IMF were reevaluated and the paraffin blocks were further sectioned, stained for H & E and reticulin and Perl’s reaction. The patients were classified into four groups according to Cologne criteria for which both subjective and quantitative evaluations of trephine biopsies were performed. Results: Both qualitative and quantitative evaluation was performed on trephine biopsies. The most consistent finding with progression of diseases was megakaryocytic and granulocytic proliferation with preponderance of megakaryocytes. The study also proves that most patients showed an increase in the number of hemopoietic cells, reticulin fibers, trabecular bone width, osteoblastic index, blood vessels, and a reduction in the iron stores. Conclusion: A thorough assessment of bone marrow biopsy including adequate tissue sampling stained for reticulin and iron stain are essential for the diagnosis and staging of idiopathic myelofibrosis. Keywords: Idiopathic myelofibrosis, trephine biopsy, subjective, quantitative
Keywords
Idiopathic myelofibrosis; trephine biopsy; subjective; quantitative

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