Optimal Conditions For Production Acidic D-Xylanase By Aspergillus niger in Submerged Fermentation

Omar Abdulatif, Muhamed; H. Assmaeel, Hyder; Kadhim Obeid, Raghad; Adnan Abbas, Ayat

	Optimal Conditions For Production Acidic D-Xylanase By Aspergillus niger in Submerged Fermentation
Jornal of Biotechnology Research Center
Article 1, Volume 5, Issue 3, 2011, Pages 14-21
Authors
Muhamed Omar Abdulatif; Hyder H. Assmaeel; Raghad kadhim Obeid; Ayat Adnan abbas
Abstract
he Xylanase producing strain Aspergillus niger was isolated from soil on potato dextrose agar in the presence of xylan as its first substrate for primary isolation, and then grown under liquid medium fermentation in the presence of crude xylan (rice husk) to produce D-Xylanase. the optimum conditions were determined as follows: the Optimum pH for xylanase production was found pH 5.0, xylanase was induced by xylan (rice husk) 0.1% and the production was (61.221 U/ml) and nitrogen source Yeast extract recorded highest enzyme production( 89.71 U/ml), and repressed by carbon source xylose the highest enzyme production (88.69 U/ml). The optimum temperature was 40°с for xylanase production was (35.15 U/ml), the optimum period after 7 days of incubation was (52.33 U/ml) ,the optimum substrate concentration 0.1% was (45.95 U/ml), and the optimum inoculum size was 1 x 106 (spore /ml) recorded (57.19 U/ml ).

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