RESPONSE OF SOME MELON CULTIVARS TO INFECTION BY CUCUMBER MOSAIC VIRUS UNDER FIELD CONDITIONS

Al-Ani, R.A.; Sabir, L.J.; Adhab, M.A.; Hassan, A.K.

	RESPONSE OF SOME MELON CULTIVARS TO INFECTION BY CUCUMBER MOSAIC VIRUS UNDER FIELD CONDITIONS
The Iraqi Journal of Agricultural Science
Article 1, Volume 40, Issue 6, 2009, Pages 1-8
Authors
R.A. Al-Ani; L.J. Sabir; M.A. Adhab; A.K. Hassan
Abstract
This study was conducted to test the response of melon cultivars, Local1 , Local2 , Local3 , Local4 , Local5 , Local6 , Local7 , Local8 , Local9 , Ananas 2nd generation1 , Ananas 2nd generation2 , Emerlad to cucumber mosaic virus (CMV). The virus was isolated from infected melon plants, and identified by mean of symptoms on indicator plants, aphid transmission, and immunostrip test. Chenopodium murale was responded to inoculation by the virus by necrotic local lesion on inoculated leaves, Ch. amaranticolor by chlorotic local lesions on inoculated leaves, Datura stramonium by small chlorotic local lesions on inoculated leaves, developed later to mosaic. Cucurbita pepo was responded to the infection by the virus by chlorotic local lesions on inoculated leaves developed to mosaic on the other leaves, and Lycopersicon esculentum by vein clearing, yellowing of leaves and filliform shaped. The immunostrip specific for CMV showed positive reaction with extracts from infected melon plants, negatively with extracts from healthy melon plants. Melon cultivars were cultivated in the field of Plant Protection Department, College of Agriculture, exposed to the natural infection by CMV from infected cucumber plants infested with the vector Myzus persicae, posed at different sites of the field. 2 weeks after cultivation. Samples from melon cultivars were taken after one month and submitted to virus detection by bioassay on cucumber plants, immunostrip test, and transmission by aphid. Symptoms of mosaic was manifested on cucumber leaves mechanically inoculated by extracts from Local1 , Local2 , Local3 , Local4 , Local6 , Local7 , Local8 , Local9 , Ananas 2nd generation2, or exposed to aphids fed on these cultivars. Also, results of immune strip test showed the presence of CMV in these cultivars. No symptoms were observed on test plants when mechanically inoculated with extracts from Local5 , Ananas 2nd generation1 and Emerlad, or exposed to aphids fed on these cultivars. No reaction observed by CMV immunostrip with extracts from these cultivars. This indicates that these cultivars were resistant to CMV.

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