ANTIMICROBIAL ACTIVITY OF HIBISCUS SUBDARIFFA EXTRACT ON SOME MICROORGANISM.

M. Hakkem, Ibtehaj.; M. Awda, Jasim.; Q. Hameed, Hiba.

	ANTIMICROBIAL ACTIVITY OF HIBISCUS SUBDARIFFA EXTRACT ON SOME MICROORGANISM.
The Iraqi Journal of Agricultural Science
Article 1, Volume 39, Issue 6, 2008, Pages 134-141
Authors
Ibtehaj. M. Hakkem; Jasim. M. Awda; Hiba. Q. Hameed
Abstract
Inhibition activity of water extract and flavonoid extract of dried and locally produced Hibiscus subdariffa on some microorganism was studied concentrations of 1000, 2000 and 3000 ppm was used against Bacillus subtillis, Escharichia coli Pseudomones spp., Saccharomyces spp., Kluveromyces spp. , Candida spp. , Aspergillus niger, Trichoderma spp. and Penicillium spp. Flavonoid extract of Hibiscus subdariffa showed highest lnhibition activity aganist the above mentioned microbes as compared to water extract. Filter paper disc diffusion was used to detect the inhibition activity of Hibiscus subdariffa extract against bacteria and yeasts. B. subtilis was the most sensitive to the flavonoid and water extract with all concentrations , followed by Pseudomones spp. E.coli was less affected by both extracts. The yeast Kluveromyces spp, was the most sensitive to the water extract in comparison to Sacchromyes spp. , while Sacchromyces spp was more sensitive to flavonoid extract than Kluveromyces spp. Both extracts had no effect on Candidia spp. at all concentrations. Poisoned food technique was used to detect the inhibition activity against the molds. A. niger was the most sensitive to both water and flavonoid extracts and at all concertrations , followed by Trichoderma spp, while Pencillium spp. was the least affected by flavonoid extract and its growth was not inhibited by water extract even ander higher concentration.

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