EFFECTS OF AFLATOXIN B1 ON SOME SKELETAL MUSCLE RESIDENT CELLS USING A NUCLEAR DIFFERENTIATING STAIN TECHNIQUE

F. Al-Habib, May

	EFFECTS OF AFLATOXIN B1 ON SOME SKELETAL MUSCLE RESIDENT CELLS USING A NUCLEAR DIFFERENTIATING STAIN TECHNIQUE
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 5, Issue 2, 2007, Pages 71-77
Author
May F. Al-Habib
Abstract
Background:Aflatoxins are one of the toxigenic fungi that draw attention for researcher, they are a group of closely related mycotoxins that can contaminate food. The problem of using contaminated food with toxigenic fungi is still one of the most important stigmas in the field of nourishment of human and animals. Objectives:This study was designed to determine how Aflatoxin B1 contaminated food and feeding regimen might affect and induce specific changes in the muscle resident cells. Methods:Two groups of animals were studied one fed with Aflatoxin B1 contaminated food and the other fed with Aflatoxins free diet. Rats were fed daily with diet contaminated with the spore. The Extensor digitorum longus muscle was removed and cut into small pieces and prepared by the method of Torikata (1988). Semi thin sections were obtained and stained by a nuclear differentiation stain. Results:Animals treated with Aflatoxin B1 have shown a marked increase in body weight. Aflatoxin B1 showed pronounced effects on muscle nuclei and on the vascularity of skeletal muscle fibers. Conclusions:lt has been concluded that AFB1 have marked effects on the number of cells found in skeletal muscles. Keywords: Aflatoxins B1- Skeletal muscle- nuclear differentiating stain
Keywords
Aflatoxins B; Skeletal muscle; nuclear differentiating stain

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