| This study was carried out to survey the mycroflora of stored maize . Special attention was paid to Fusarium moniliforme (Sheldon) invasion percent of its mycotoxin Fumonisin B1 occurrence .Microbial and chemical analysis detection that Fumonisin B1 (FB1) on three places in Baghdad , Babylon and Kut.The result showed that maize kernels were discolored in rate of between 28% in Baghdad and 42.6 in Babylon, and means 32.98 , 34.4 and 39.8 in Baghdad , kut, Babylon in respectively. While, Microbial analyses showed associated fungi were Alternaria alternate, Aspergillus Spp. Fusarium graminiarum , F. moniliforme, Mucor sp., Penicillium spp and Rhizopus sp. The F. moniliforme was found to be the most dominant species with infection percent of 61%followed by Aspergillus , Penecillium , Rhizopus , Mucor and Alternaria species . On the other hand 41.6% of examined F. moniliforme isolated were found to be naturally contaminated maize samples and more than isolated in Babylon that 55.5% , While kut isolated that 16.6% of the examined Maize Samples.The chemical analysis revealed that 44.4% of the examined maize samples were Fumonisin B1 with contaminated .Jimsonweed ( Datura stramonium L. ) leaves were sensitive to the toxic culture of the F. moniliforme isolates ( Fumonisin B1 producers ) as bioassay tools . All the examined maize cultivars of this study were sensitive to F. moniliforme invasion and Fumonisin B1 occurrence under field conditions. In general the result of this stady proved that responsibly mean Controll With Maize cultivars they found to be of cultivars 5012 more Sensitivity than Cultivars , and the results also Showed 106 Cultivar lest effect in inoculation . |