STUDY THE INHIBITING EFFECT OF PRECIPITATED PROTEIN OF CURCUMA IN BOTH GRAMS NEGATIVE AND POSITIVE BACTERIA

	STUDY THE INHIBITING EFFECT OF PRECIPITATED PROTEIN OF CURCUMA IN BOTH GRAMS NEGATIVE AND POSITIVE BACTERIA
AL-TAQANI
Article 1, Volume 24, Issue 4, 2011, Pages 0-0
Abstract
The aims of the study is to identify the inhibition activity of precipitated protein of Curcuma root plant on a group of bacteria, both Gram negative and positive such as: E. coli, Proteus vulgaris, Corynebacterium pyogenes, Klebsiella pneumonia, Bacillus cerus and Staphylococcus aureus. Precipitated protein was obtained from Curcuma root plant from a specified place in Mosul city, and was classified in the college of science/ Department. of Biological Sciences. Isolates are obtained from specialized Lab. And Al-Khansa hospital Lab. In Mosul city. All isolates were checked by many diagnostic biochemical tests. E.Coli is Gram negative, non-motile, non-spore forming, positive for glucose fermentation, negative to urease, nitrate, methyl red, voges proskauer, H2s, production tests. Proteus vulgaris, Gram negative, positive for glucose fermentation, urease, H2s, production tests, negative to citrate, indol tests. Klebsila pneumonia is a Gram negative, non-motile, positive for glucose fermentation, catalase, citrate, urease, voges proskauer tests, and negative for indol, methy red tests. Staphylococcus aurese, Gram positive, non-motile, non-spore forming, and is arranged in clusters like groups, positive for glucose fermentation, urease, nitrate production tests, and it is negative to oxidase, indol tests. Cornyebacterium pyogenes, Gram positive, non-motile, non-spor forming, pleomorphic bacteria, positive for glucose fermentation, and negative for oxidase, indol, catalase, nitrate methyl red tests. Bacillus ceruse, Gram positive, positive for glucose fermentation, Nitrate, citrate, negative for urease test. In this study Kirby Bauer method was used to identify the inhibitory effect of precipitated protein. For sterilization, (1) gram of the precipitated protein was dissolved in (5) ml of dimethylsulphoxid and the mixture was sterilized by pasteurization in (62) c˚ for (15) minutes, and 1 cm of the sterilized mixture was added to a vial containing (100) discs, and was fixed on Miller Henton agar, then was incubated at 37 c˚ for 24 hours. Measuring the inhibition zone growth, the inhibition zone was different according to the type of bacteria. The higher effect of inhibition was observed on E. coli, Proteus vulgaris, Bacillus cerus, Staphylococcus aureus، the precipitated protein showed less inhibition effect on Klebsiella pneumonia than the previous types, and the precipitated protein didn't show inhibitory effect on Corynbacterium pyogenes when compared with the control sample which contained antibiotics, Ciprofloxacin (5) mg, it is the preferred , and Gentamycin (10) mg.

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