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A STUDY ON HUMAN PAPILLOMAVIRUS USING IN SITU HYBRIDIZATION TECHNIQUE AND ITS ROLE IN CERVICAL NEOPLASIA | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 6, Issue 1, 2008, Pages 28-37 | ||
| Authors | ||
| Arwa Mujahid Al-Shwaikh; Ismail Ibrahim Latif; Liqaa R. Al Khuzaee | ||
| Abstract | ||
| Background: clinical epidemiological studies have shown that human papillomaviruses play major role in the development of different types of cervical lesions, are therefore considered as the major infectious etiological agents of genital lesions and cancer. Objective: to determine the prevalence of HPV DNA by using in situ hybridization technique among archival tissue specimen of the uterine cervical lesions and normal cervical postmortem tissue biopsies. Material & Methods: Eighty cervical tissue samples were included in this study. 70 archival tissue biopsy samples comprised a risk group for HPV infection and / or cervical neoplasia; these were selected for the years from 1998 to 2005 from histopathology files of Al-kadhimiya teaching hospital, Al-Ilwiya teaching hospital, Al-Yarmouk hospital, Medical city department of teaching laboratories, and from four private laboratories. The patients mean age was 43.1 years with a range of 20 to85 years. The remaining 10 normal cervical postmortem tissue biopsies were obtained from the institute of forensic medicine and considered as control group. These autopsies were taken from virgin female cervices, their mean age 23.1 years with a range of 18-30 years. In Situ Hybridization was performed for the detection of HPV on cervical tissue. Results: All normal control cases showed no specific signals for HPV DNA. 6 (30%) of 20 cases of cervical tissue with codylomatous changes, 1 (11.11%) of 9 cases of CIN I.3 (21.43%) of 14 cases of CIN II/III, and 9 (33.33%) of 27 cases of ISCC were shown to be positive for HPV 6/11/16/18/31/33 DNA. Conclusion: The In situ hybridization enabling direct visualization of viral tissue distribution and better substantiate HPV as a causal agent in cervical neoplasia.A significant association (p <0.05) was found between Insitu Hybridization signal pattern and the histological type of cervical noeplasia. | ||
| Keywords | ||
| Human Papilloma virus; cervical intra epithelial neoplasia; cervical carcinoma; in situ hybridization technique | ||
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