DETECTION OF IL-8, IL-10 AND IFN- Γ mRNA IN TROPHOBLAST TISSUES OF RECURRENT SPONTANEOUS ABORTION USING IN SITU HYBERDIZATION

Hussein, Amal; Abdul Mohymen, Nidhal

	DETECTION OF IL-8, IL-10 AND IFN- Γ mRNA IN TROPHOBLAST TISSUES OF RECURRENT SPONTANEOUS ABORTION USING IN SITU HYBERDIZATION
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 6, Issue 1, 2008, Pages 121-127
Authors
Amal Hussein; Nidhal Abdul Mohymen
Abstract
Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL-10, have been shown to exert deleterious effects on pregnancy, inhibiting fetal growth and development . Aim: Measurement of the locally concentrations of selected Th1 and Th2 cytokines in women with a history of recurrent spontaneous abortion (RSA) at the time of abortion using in situ hyberdization technique. Methods: A total of one hundred and nineteen women, ranged from the mean age (23.9 − 28.5)years, were enrolled in the current study and were further classified into three categories: Group A- Recurrent spontaneous abortion (RSA): n= 62 women, with a mean age of (28.5 + 0.68);Group B- non- recurrent spontaneous abortion (non-RSA): n= 34 women, with a mean age of (26.4 ± 0.85)and group C- Control (successful pregnancy): n= 23 women, with a mean age of (23.9 ± 0.88). From each patient and control, placental tissues were collected. Trophoblasts tissues (an image for the local microenvironment) were screened to determine their in situ levels of IL-10 and IFN-γ based on cDNA probes (for in-situ hybridization, ISH). Results: There was a significant increase in the level of IL-10 within trophoblast tissues biopsies exclusively from women with successful pregnancies (group C) (p < 0.001). On the other hand, IFN-γ was found predominantly expressed in trophoblast tissue biopsies of patients with RSA whether IHC or ISH were conducted (p< 0.05). Accordingly, only trophoblast tissues biopsies from patients with RSA revealed a significant increase in the ratio of IFN-γ/IL-10 levels expressed as determined by in situ hybridization in comparison to the same ration calculated from trophoblasts tissues of women with successful pregnancies (group C) (p< 0.001). as marker for Th2 immune response, during successful pregnancies. Furthermore, the current study failed to demonstrate a significant difference in the tissue levels of IL-8 between RSA and control group (p> 0.05) and no significant different between non-RSA and control (p>0.05) , (always p < 0.05). Conclusion: These outcomes may further support the possible exisance of an immune response that orchestrates abortive phenomena and the possible protective role of IL-10.
Keywords
Recurrent spontaneous abortion; in situ hybridization; ISH; cytokine; IFN

Statistics Article View: 30 PDF Download: 12