Effect Alkaloid and aqueous extraction of Convolvulus Scammonia on microtubules of CHO cell line(china hamster)

T.A, Zenia; Hade, Ibrahim

	Effect Alkaloid and aqueous extraction of Convolvulus Scammonia on microtubules of CHO cell line(china hamster)
Diyala Journal For Pure Science
Article 1, Volume 7, Issue 3, 2011, Pages 48-58
Authors
Zenia T.A; Ibrahim hade
Abstract
The study evaluated the effect of selected on crude Alkaloid and aqueous extraction from roots of ( Convolvulus Scammonia) the microtubule network of CHO cell line (china hamster) GFP tubulin labeled and the recovery of its disruption. Model experiments were used for demonstration of the quantification of microtubule network changes induced by crude alkaloids using computer-assisted image analysis. Cells were treated with Alkaloid and aqueous extraction from roots of (Convolvulus Scammonia) at various concentrations from 2 μg/l to 800 μg/l for 60 min, or with crude alkaloid at a concentration of 4615 μg/l and 9230 μg/l for 60 min. Microtubules were detected by means of indirect Immunofluorescence. The damage was examined in a fluorescence microscope. Also, cells were treated for 60 min with alkaloid at concentrations of 20 μg/l or 800 μg/l and the recovery process was studied in time intervals of 6, 7, 8, 9, 10 hours, or 8 and 12 hours, respectively. Differences in the arrangement of microtubules were assessed by means of quantification of the cytoskeleton changes in cells treated with alkaloid at a concentration of 20 μg/l and in untreated control cells.
Keywords
Effect Alkaloid; aqueous extraction; Convolvulus Scammonia microtubules

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