Study the Effect of Enterocin produced by Enterococcus faecalisU36 Against Some Food Borne Pathogenic Bacteria

Kandala, NuhaJoseph; M. Aziz, Ghazi; Khanaka, Hussein

	Study the Effect of Enterocin produced by Enterococcus faecalisU36 Against Some Food Borne Pathogenic Bacteria
Jornal of Biotechnology Research Center
Article 1, Volume 4, Issue 1, 2010, Pages 54-64
Authors
NuhaJoseph Kandala; Ghazi M. Aziz; Hussein Khanaka
Abstract
Enterocin U36 (ENT U36), is a bacteriocin produced by Enterococcus faecalisU36, strain isolated from urine samples have collected from patients suffering from urinary tract infections. The Bacteriocin is an antimicrobial proteins or peptides that inhibit growth of bacteria closely related to the producing organism. The results has been shown that ENT U36 active against Enterococcus faecalis S10 Lactococcuslactis,Lactobacillusfermentumand few other food borne gram positive pathogens bacteria including Listeria monocytogenes, Staphylococcus auraus,Bacillussubtilis and Streptococcusspp . The inhibitory type and mode of action of purified ENT U36 were tested against some isolates test bacteria which includedL.monocytogenes and E.coli It was observed that purified ENT U36 have bacteriostatic effect when the numbers of inhibited test bacteria increased in parallel with the increased activity of ENT U36 (unit/ml) . The treatment period also has its effect on reducing the numbers of test bacteria. The results showed that 400 unit/ml was enough to kill L.monocytogenes viable count of in 3.1 x105 cell/ml in 2 hours when treated with ENT U36, and E.coli in 3.7x105 cell/ml in 24 hour'. And when lytic activity of purified ENT U36 studied, it was observed that its mode of action was on cell membrane and did not show any lytic activity on the isolated cell walls of test bacteria or any lytic activity on DNA. This beneficial trait has led to utilization of purified enterocinas as food additives.

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