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In vitro Effect of Actiavted Charcoal and microcutting type on microtuberization of potato Cv. Gardenal | ||
| AL-TAQANI | ||
| Article 1, Volume 24, Issue 1, 2011, Pages 83-90 | ||
| Abstract | ||
| Tips of sprouts (0.3 – 0.5 cm) produced from the tubers of potato cultivar Gardenal were washed with tap water and disinfected with NaOCl then cultured onto proliferation medium.The cultures were incubated for 35 days at 16 hours photoperiod at 25 ± 2 ºC under 1000 lux light intensity. In vitro – proliferated shoots were cut into1 ـ 2 cm length three types of microcuttings ( i.e apical, subapical and basal ) were cultured onto Murashige and Skoog ( MS ) medium suplimented with 8% sucrose for microtuberization. Activated Charcoal treatments (0.0, 0.5, 1.0, 1.5, 2.0, 2.5 or 3.0 g /l) were added to the microtuberization medium.The weihgt, size, and eyes number of microtubers were significantly increased when basal microcuttings were cultured on microtuberization medium supplimented with (2 g/l) charcoal while (2 g/l) charcoal with the subapical cuttings resulted in high proliferation rate. | ||
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