IMMUNOPHENOTYPHING OF PERIPHERAL BLOOD LYMPHOCYTES TO PERSON EXPOSED TO ELECTROMAGNETIC FIELDS

Abdul -Wahid, Rafid

	IMMUNOPHENOTYPHING OF PERIPHERAL BLOOD LYMPHOCYTES TO PERSON EXPOSED TO ELECTROMAGNETIC FIELDS
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 7, Issue 2, 2009, Pages 50-58
Author
Rafid Abdul -Wahid
Abstract
Background: There is considerabl evidence relating electromagnetic fields (EMFs) exposure to reduce immune system competence .and these changes associated with cell growth control, differentiation and proliferation of cells of immune system, trans membrane signaling cascades, gap junction communication, immune system action. Objective: to investigate the Phenotyping of peripheral blood lymphocytes of volunteer’s exposed at least 10 years to electromagnetic fields (EMFs) induced by transmission power lines in their residential area , Subjects and methods: fourty five volunteer’s aged between 25 and 65 Years, exposed for at least 10 years to electromagnetic fields (EMFs) induced by transmission power lines in their residential area and Fifteen male of similar age unexposed, away from the transmission power lines as a control group were used in this study . The electromagnetic fields (EMFs) (with range of 50 Hz) beside the homes of the volunteer’s. This study carried out in three different are as of Baghdad included (Al –Bladyat, Hay Al-Adel and Al-Dorra cities) , The groups of this study were divided into three sub- groups according to the distance away from the towers of transmission power lines (1) range: from 1 to 25 meter (2) from 25 to 50 meter (3) from 50 to 75 meter . Phenotyping of peripheral blood lymphocytes had been done by direct immunofluorescent microscopy using anti –CD 3(for T-cells detection), anti-CD4 (for T- helper-cells), anti CD8 (for T-cytotoxic/suppressor cells), anti CD21 (for B-cells) and anti CD56 (for natural killer cells). Results: A statistically significant reduction of PBL percentage bearing CD3,CD4,CD19,CD56(P<0.01) between the exposed volunteers and control ;.except CD8 which showed no significant different between these groups . the mean percentage of CD4+/CD8+ ratio in exposed volunteers groups was significantly (P<0.01) lowered in comparison to control group . Conclusion: We postulated that the chronic exposure to electromagnetic fields from power lines caused suppression in immune system. Keywords: Electromagnetic felid, CD4, CD8, CD21, CD56, Lymphocytes, phenotyping
Keywords
Electromagnetic felid; Lymphocytes; phenotyping

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