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MORPHOMETRIC STUDY ON THE Ag-NOR CHANGES IN SKELETAL MUSCLE RESIDENT CELLS WITH AGING | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 7, Issue 2, 2009, Pages 109-115 | ||
| Authors | ||
| Huda Rashid Kareem; May Fadhil Majid Al-Habib | ||
| Abstract | ||
| Background: Aging is the deterioration of mature organism resulting from time dependent irreversible changes. The effects of aging on skeletal muscle cells have not been much-elucidated using Ag-NOR analysis. Objectives: This study aims to demonstrate the effects of aging on Ag-NOR in morphometric & counting aspects. Materials and methods: The Extensor digitorum longus muscle of forty Albino male rats with age ranging from 27 days up 18 month were studied. Paraffin blocks were performed & sectioned. Ag –Nor stained sections were de-waxed, rehydrted, developer solution was used. Morphometric Analysis of the Ag-NOR stained nuclei through using a soft wear GLI (Global lab image 2) with a microscope connected to PC Unit, a software used to analyze the picture that seen through the microscope, nuclear area, nuclear perimeter, and roundness were calculated,& counting of the number of Ag-NOR stained nucleoli per stained cells. Results: In neonate age group, the nuclei have high affinity to the stain. High proportion of nuclei can be recognized, with the higher count of Ag-NORs per cells. • In adult age groups the affinity to the stain is reduced, the nucleus appears to have smaller count of Ag-NORs per cells. • In old age group the staining intensity seem to be highly reduced, the nucleus seem to have single, rounded Ag-NOR. Conclusion: A significance differences is seen in Ag NORs in cells of skeletal muscle fibers with aging demonstrated by counting and morphometric method of Ag-NOR analysis. Key words: Skeletal muscle, Ag NOR. Morphometry. | ||
| Keywords | ||
| Skeletal muscle; Ag NOR; Morphometry | ||
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