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Effect of Non Genetic Factors on Arabi ewes Oocytes Maturation, and in vitro Fertilization the | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 12, Issue 2, 2012, Pages 8-13 | ||
| Author | ||
| Khalaf Abdulrazzaq Hasan Al-Rishdy | ||
| Abstract | ||
| The study was conducted at Assisted Reproductive Technology Center (A R T) College of Medicine, University of Basra, from January, 2009 to January,2010 . The aim was to study the ability of in vitro fertilization of Arabi sheep Ocolytes and sperms capacitation to increase their activity. A total of 4558 ova were collected from ovaries obtained from Basra Slaughter House to study the effect of month, season, follicle diameter and culture media on maturing sheep oocyte and in vitro fertilization. Results revealed a significant (P<0.05) effect of months on Oocytes in vitro maturation . October showed highest percentage (77.97 %) .This month also showed significant increases in in vitro fertilized and divided Oocytes (55.43% and 37.25%) respectively. Seasons had significant (P<0.05) effect on in vitro maturation, fertilization and division of Oocytes. Autumn gave the highest values (75.35%, 53.68% and 34.93%) respectively. Oocyte were divided into five classes(2< , 2>-3, 3>-4, 4>-5,5>) .When in vitro fertilization was done , Oocyte with diameter >5 recorded highest (P<0.05)in vitro fertilization and division percentages (78.18 , 56.97% and 38.77%) respectively | ||
| Keywords | ||
| Non Genetic Factors; fertilization | ||
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