GENOTYPING OF HLA-CLASS-I BY PCR-SSP OF IRAQI BREAST CANCER PATIENTS

J. Al-Nema, Ameera; Ala,; Ghany Hussien, A; Abdul Muhymen, Nidhal; A .Al-Hassan, Ahmed

	GENOTYPING OF HLA-CLASS-I BY PCR-SSP OF IRAQI BREAST CANCER PATIENTS
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 7, Issue 3, 2009, Pages 5-12
Authors
Ameera J. Al-Nema; ; a Ghany Hussien; Nidhal Abdul Muhymen; Ahmed A .Al-Hassan
Abstract
Background: The aetiology of breast cancer is multifactorial, in which genetic predisposition; environmental factors, hormones and even the infectious agents are thought to interact in the manifestation of disease. In this regard, alleles of HLA are important immunogenetic risk factors, but their associations show different frequencies in different populations. Objectives: This study was established to shed light on the possible association of HLA class I alleles with BC in Iraqi female patients. Subjects and Methods: The study included 60 subjects: 30 breast cancer patients, 12 patients with benign breast lesions as first control and 18 apparently healthy subjects as second control. Polymerase chain reaction-specific sequence primers (PCR- SSP) assay was conducted to assess HLA- typing. Results: Out of 95 HLA class I alleles (A= 24; B= 48; C= 23), one allele (HLA- A03010101-07, 09-11N, 13-16 allele) showed a significant variation between breast cancer patients and control groups (healthy and disease controls) (50% vs. 16.6%, OR=5, EF= 0.40, P= 0.041), (50% vs. 8.3%, OR=11, EF=O.45, P=0.024) respectively. Conclusions: The results demonstrated that HLA- A03010101-07, 09-11N, 13-16 allele may played a role in the etiology of the disease. Keywords: Breast cancer, HLA, PCR.
Keywords
Breast cancer; HLA; PCR

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