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ESTIMATION OF PLATELET COUNT ON THE BASIS OF RED CELL: PLATELET RATIO | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 7, Issue 3, 2009, Pages 40-45 | ||
| Author | ||
| Balsam F Abid | ||
| Abstract | ||
| Background: Modern haematology analyzers are able to produce platelet counts with great precision and accuracy. However, in certain cases these analyzers produce erroneous platelet results.Therefore, the estimation of platelet count from blood smears should be syste¬matic each time the automated count is erroneous. However, in comparison with the procedure for an automated count, the examination of a blood smear using a counting Neubauer chamber is a labor-intensive and therefore relatively expensive investigation. Objective: Verification of the reliability of the estimation technique of platelet count on the basis of red cell: platelet ratio. Material and Methods: In the period between January 2006 and March 2006 one hundred platelet counts were executed in the National Center for Haematological Diseases by two laboratory methods: an automa¬ted count using an impedance cell counter and then a manual method by reviewing microscopic blood smears. The number of platelets per 1000 erythrocytes was multiplied by the automated RBC (x106 cells/µl) to give an approxima¬te manual count (x103 cells/µl). Two-paired t-test was used for comparison of the two methods. Results: Platelet count using the manual method was as follow: the range was 100-499x103/µl, the mean count was 263.11±104.07 x103/µl, and the median was 247.5 x103/µl. using the automated method, platelet count ranged between 95-484 x103/µl, the mean was 258.43 x103/µl, and the median was 242.5 x103/µl. There was no significant difference in results of platelet count using both methods (P<0.05). Regression analyses gave the following equation by comparing the automated (y) to the manual method (x): y=0.9893x - 1.8621 (r= 0.966). The paired t-test showed no significant difference between the two methods (p<0.05). The ICC was equal to 0.988. The plot of the differences between the automated and manual values against their means showed that the difference mean was 2.116 with a standard deviation SD= 40.215. It was noticed that 93% of the differences were within the agreement limits (mean±2SD). Conclusion: Red blood cell:platelet ratio method requires only an accurate RBC count performed on a calibrated hematology analyzer to calculate platelet count. This method is precise, simple, and consumes less time than using a counting chamber, and therefore, potentially should supersede ordinary manual counting. Key words: red cell: platelet ratio, platelet count | ||
| Keywords | ||
| red cell; platelet count | ||
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