GENETIC MUTATION OF PSEUDOMONASAERUGINOSA OM13 TO IMPROVE ITS PRODUCTIONOF ANTIFUNGAL COMPOUND

	GENETIC MUTATION OF PSEUDOMONASAERUGINOSA OM13 TO IMPROVE ITS PRODUCTIONOF ANTIFUNGAL COMPOUND
Iraqi Journal of Biotechnology
Article 1, Volume 7, Issue 1, 2008, Pages 117-132
Abstract
ABSTRACTThe ability of P. aeruginosa OM13 in antagonizing or inhibiting the growth ofsome phytopathogenic fungi was tested by measuring the inhibition zone for thegrowth of the tested fungi (Alternaria alternata and Rhizoctonia solani) caused bythe growth of the bacterial isolate (P. aeruginosa OM13 ) and by the action of theculture filtrate of this isolate on test fungi. It was found that this isolate inhibit thegrowth of the test fungi and the inhibition zones were 5mm, 7mm for Alternariaalternata and Rhizoctonia solani, respectively. To improve the antifungalcompound production by P. aeruginosa OM13, it was treated with mutagenic agentnitrosoguanidine (MNNG). The results showed that the production of 5 isolateswere highly increased, in which RA5 gave the highest inhibition zone and O.D520nmvalues when compared with other. An attempt to characterize the partiallypurified antifungal compound produced by P. aeruginosa OM13 by using infrared(IR), UV absorbance and Thin Layer Chromatography (TLC) were done and theresults showed that the compound appeared as a single dark spot on a TLC plateunder UV-light that had Rf value 0.32 from IR, UV analysis. It was confirmed thatthe compound was aromatic and contain aliphatic group. It can be concluded thatthe active antifungal compound might be either pyocyanine which is a derivative ofthe antibiotic phenazine or might be a new derivative of phenazine.Key words: Genetic mutation, Pseudomonas aeruginosa, production, antifungal

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