Virulence factors of enterococci species isolated from nosocomial and community acquired infections

A. Al-Duliami, Abbas; G. Nauman, Nadhum; R. Salman, Afak; SH. Hasan, Abdul-Razak

	Virulence factors of enterococci species isolated from nosocomial and community acquired infections
Diyala Journal For Pure Science
Article 1, Volume 7, Issue 3, 2011, Pages 174-182
Authors
Abbas A. Al-Duliami; Nadhum G. Nauman; Afak R. Salman; Abdul-Razak SH. Hasan
Abstract
Several virulence and pathogenicity factors have been described from enterococci that enhances their ability to colonize patient's tissues, increase resistance to antibiotics, and aggravate the infection outcome. The present study aimed to investigate virulence and pathogenicity factors among enterococci species isolated from nosocomial and community acquired infection in Diyala. The study was conducted in Baquba General Hospital and Al- Batool Hospital for Maternity and children during the period from 1st. September/2005 to 30th. September /2006. A total of 343 specimens were collected from 213 inpatients and 130 outpatients. 200 (58.3%) were females and 143 (41.7%) were males. The mean age of patients was (32.8 ± 17.2) years. 44 isolates of enterococcal species were recovered from different clinical specimens and identified according to standard bacteriological and biochemical criteria. The presence of certain virulence and pathogenicity factors, namely; gelatinase and hemolysin production, biofilms formation, agglutination of erythrocytes, presence of capsule, and adherence to epithelial cells were detected. Data were statistically analyzed. The results showed that all isolates of E. gallinarium and E. avium were biofilm former compared to 76.7% and 70% of E. faecalis and E. faecium respectively. Furthermore, all isolates of E. gallinarium and 76.7% of E. faecalis were β-lactamase producer. Additionally, all isolates of E. avium and 76.7% of E. faecalis were agglutinated RBCs. The presence of capsule was highest among E. faecalis isolates (26.7%). The results also revealed that all E. galinarium and E. avium isolates were non-hemolytic. Furthermore, among 12 isolates which express β- hemolysis, 10 (33.3%) and 2 (20%) were E. faecalis and E. faecium respectively. α-hemolysis were found among 10 (26.7%) isolates of E. faecalis and 2 (20%) isolates of E. faecium. It can be concluded that Local isolates of enterococci species recovered from different clinical specimens are multi-virulence bacteria.
Keywords
KEYWORDS; Multiple; drug resistance; virulence factors

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