Microbial Contamination in the Surgical Theaters in Baquba General Hospitals

T.A,; Mohammed,

	Microbial Contamination in the Surgical Theaters in Baquba General Hospitals
Diyala Journal For Pure Science
Article 1, Volume 6, Issue 1, 2010, Pages 227-245
Authors
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Abstract
Medical contamination in the operation surgical theaters is considered a major and a very important cause of acquired nosocomial infections. The objective of this study was to assess the incidence of microbial contamination in the operation surgical theaters in Baquba General Hospital in Diyala province, and the source of the probable contamination, so (700) swabs were collected from four different operation theaters, swabs were taken in duplicate from different surfaces, equipment, and antiseptic solutions before and after surgical operation, cultured on different cultured media, culture characteristics were examined; isolates identification was confirmed by biochemical tests. During the period of this study (April- June- August) incidence of positive cultures was 17.71%, 13.71%, and 12% respectively, with a statistically significant increase (P> 0.05) in April, where the total gram negative bacterial isolates was 57.25% ,56.25%,and 78.57% respectively with a statistically significant increase (P> 0.05) along the study period . Also, the total gram positive bacterial isolates was 38.7%, 39.57%, and 20.23% respectively, whereas the Candida albicans was recorded the following percentages of isolation 4.03%,4.16%,and 1.19% respectively with a statistically significant increase (P> 0.05) in April .Coliforms were given the highest isolation percentages 35.45%,37.48%,and 42.85% respectively. E.coli, the most representative pathogen of coliforms was the most common isolated pathogen by reaching 17.74%, 20.83%, and 23.80% respectively. Staphylococcus epidermidis was ranked the second in April and June, recording 29.83%and 27.08%respectively, whereas Pseudomonas aeruginosa was placed in the second rank in August recording 23.80%. Generally, a clear decrease in the percentage of bacterial and fungal positive cultures was marked towards summer months, with an obvious prominence of the gram negative bacteria, also the results that concerning distribution of different microbial isolates in June and August were showed to a great extent the same pattern of microbial distribution which observed in April.
Keywords
Nosocomial infections; Incidence; source of infection; types of microbial infections

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