USE OF TEETH SAMPLES FOR GENDERDETERMINATION BY PCR IN IRAQI CASE

	USE OF TEETH SAMPLES FOR GENDERDETERMINATION BY PCR IN IRAQI CASE
Iraqi Journal of Biotechnology
Article 1, Volume 9, Issue 4, 2010, Pages 819-827
Abstract
ABSTRACTIdentification by DNA analysis has proven valuable when the use of traditionalforensic identification methods such as fingerprints or dental radiographs is difficultor impossible in situations such as explosions. Identification is even more difficultbecause human remains are often fragmented and may be commingled. Teeth are auseful source of DNA and can often survive extreme environmental conditions. DNAwere extracted from 10 male and 10 female teeth samples ranging in age from 24 to 47year subjects for dental treatments were used as material that contains theAmelogenin gene(amel), which is the key of sex determination in our study .PCRreaction was performed for a series of dilutions prepared from 10ng DNA to assessthe minimal amount of DNA required for PCR amplification which is the primaryconcentration of the extracted DNA from dental materials of all 20 samples. The PCRproducts of the 1:100 DNA dilutions which is consider the optimum concentration.The polyacrylamide gel showed two bands with molecular weight (218bp and 212bp)for male samples and one band with molecular weight (212bp) for female samples.The results shown that the amel gene serves as a good marker for sex determination inthe Iraqi population and the PCR-based method was sensitive and proved to besuccessful for sex determination with a complete specificity.Key words: Gender determination, PCR, Amelogenin, Teeth.*To whom correspondence should be addressed (E-mail:amgb772005 @yahoo.com)
Keywords
Gender determination; PCR; Amelogenin; Teeth

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