- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Antiproliferative effects of porins extracted from Klebsiella pneumoniae on HL-60, NIH/3T3 and Human foreskin fibroblast cell (HFFC) lines measured by ELISA method | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 4, Issue 1, 2010, Pages 28-35 | ||
| Authors | ||
| ; Amir H. Al; Essam F. Al-Jumaily; Nidhal Abdulmohymen | ||
| Abstract | ||
| Approximately, 50% of the dry mass of the outer membrane of gram-negative bacteria consists of proteins, and more than 20 immunochemically distinct proteins (termed outer membrane proteins [OMPs]) have been identified. An identified local strain of Klebsiella pneumoniae was used as a primary source for the isolation and purification of porins. Multiple concentrations of purified porins (5, 10, 15, 20, 25) g/ml were incubated with three different cell lines for (24, 72 , 120) hrs, after the end of the incubation periods, the cells were treated with Cell proliferation ELISA, BrdU (colorimetric) kit to evaluate the antiproliferative effects of porins. The results revealed that porins are potent antiproliferative agent in a time and concentration dependent manner and thus could greatly affect prokaryote-eukaryote interaction as well as the whole inflammatory process resulted after infection with gram negative bacteria. | ||
|
Statistics Article View: 70 PDF Download: 50 |
||