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IN SILICO STUDY FOR PREDICTION OF DRUGTARGETS IN HELICOBACTER PYLORI | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 4, 2010, Pages 753-769 | ||
| Abstract | ||
| .ABSTRACTIn Silico Study, was conducted to search and predict essential biotargets whichmight be as drug targets in Helicobacter pylori. Gene sequences (Protein sequences)of the bacterium and its host (Homo sapiens) were retrieved from the Database forEssential Genes (DEG) (http://tubic.tju.edu.cn/deg/).The retrieved sequences were subjected to alignment process using BLASTP2.2.23+(http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=blastn&BLAST_PROG_DEF=megaBlast&BLAST_SPEC=blast2seq ) withdifferent bioinformatic parameters and different matrices such asPAM70,BLOSUM62 at different levels of E-values "0.001, 0.01, 0.05" to estimatethe essential bacterial genes that non-homologous with the human. Results showedthat 55 genes out of 323 essential bacterial genes have no homology with humanessential genes (118). The products of these genes (55 genes) were subjected toprograms to estimate the cellular location such as PSORTb v.3.0(http://www.psort.org/psortb/index.html) and TMHMM(http://www.cbs.dtu.dk/services/TMHMM/). The two programs showed that 22%of non-homologous genes (12 protein) were associated with cell membrane, 24%(13 protein) had unknown location, and the rest 54% (30 protein) were found inthe cytoplasm. It could be predicted that the membrane proteins might be goodtargets for vaccine development and as well for antibiotics affected the cellmembrane. | ||
| Keywords | ||
| Helicobacter Pylori; Drug Targets; Bioinformatic | ||
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