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Induction of Superovulation and Invitro and Invivo Fertilization of Oocyte in 6-8 Weeks old Iraqi Goat | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 2, Issue 1, 2009, Pages 9-12 PDF (67.31 K) | ||
| Authors | ||
| A. F. ALwan; J. I. Asaad; H. Abd- Alzahra; W. Q. S. Ali | ||
| Abstract | ||
| Twelve (12) female kids 6-8 weeks old were i/m injected daily with 12 mg progesterone for 18 days . Six kids were injected with 400 I.U. eCG(PMSG). hormone 48 hrs before last progesterone injection . While the other six kids were i/m injected with 100, 75 and 50 I.U of Pluset ( FSH and LH hormones). respectively, at day 13,14 and 15 of progesterone treatment, those kids were showed atypical signs of estrous. The first and second surgical operation were done 48 and 120 hrs after last progesterone injections. During first operation each ovary of kid treated with eCG. contain 3-7 follicles , while 5-10 folloicles were counted on ovaries of kids treated with Pluset. Follicles on left ovaries were aspired for invitro maturation. Fresh active 800 million sperm were injected in uterus of each kids for invivo maturation. Three Oocyte out of 13 aspired follicle were matured for e C G kids and 4 out of 15 follicles were maturated for Pluset group. During 2nd operation the ovaries of pluset kids were found to look like net of bee and honey comb and not contained any corpus luteum. Superovulation Oocyte collections and ivtro and invivo maturation could preformed ,but it is butter to used 3-4 months young female kids | ||
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