- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Study the effect of some factors in the induction of callus from the plant beans | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 6, Issue 4, 2010, Pages 209-226 | ||
| Author | ||
| Satar A.S | ||
| Abstract | ||
| The best microspores developmental stage was determined by adopting a new rapid taining method using orcein- HCl stain. It was found that the third stage was better the stage for callus induction. For anther culture conditions the pretreatment temperature (3Cْ) was applied for 1-13days,basal MS media were used as liquid ( with or without ficoll ) and solid (with 0.8% agar or 0.5% agarose ), hormones with BA: 0.5 and 1mg/l and IAA: 0.1,0.5 and1mg/l) or without hormones, activated charcoal (0.1,0.3 and 0.5%), colchicines(25 mg/l),too different concentration from sucrose [3,4,5,6%].Casein hydrolysate , amino acids and lighting conditions full darkness and light (white and red). The result showed that the pretreatment temperature gave less callus induction than the control treatment. The solid media gave better callus induction than liquid media especially when agarose was used. Whereas activated charcoal treatment and colchicine (added to the media) did not induce callus formation and the sucrose (4%) gave better callus induction (33.3%). The results also revealed that the red light gave callus induction (5%) better than the white light and darkness. | ||
| Keywords | ||
| Study the effect of some factors in the induction of callus from the plant beans | ||
|
Statistics Article View: 88 PDF Download: 15 |
||