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Study the efficiency of the enzyme glucose Oazomez product from the local isolation of the bacterium Streptomyces sp. HM5 in converting glucose to fructose | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 6, Issue 4, 2010, Pages 250-271 | ||
| Authors | ||
| Basil kamil Dalali; Mohammad Omar Mohiuddin; Aboud Hamid Jabr | ||
| Abstract | ||
| used for producing High Fructose Corn Syrup (HFCS)for a long time ago . the enzymatic method is better than chemical method for producing HFCS . There are some organisms such as Streptomyces can produce this enzyme. The aim of this research is to study the efficiency of glucose isomerase produced from local isolate of Streptomyces by determination of the conversion of glucose to fructose in three sources (Corn syrup; date syrup and standard glucose). Three samples were used as a substrate glucose corn syrup, date syrup and standard glucose solutions. Partial purified enzyme (glucose isomerase) produced from Streptomyces sp. HM5 was also used. Total and reduced sugars were determined by spectrophotometric method, specific rotation and TLC technique. The optimizations of time, substrate and enzyme concentrations were also studied. The results indicates that the optimum time for conversion glucose to fructose was 35 – 40 hours in three substrats . The optimum concentration of glucose corn syrup, standard glucose and data syrup were 30; 20 and 10% respectively. The optimum volum of enzyme is 0.5 ml with specific activity equal to 10. 80 U/ ml. The applied study shows that can be convert glucose to fructose in glucose corn syrup by using glucose isomerase produced from Sterptomyces sp. HM5 and the product can be use in many foods industries because of its higher sweeteners with low cost. | ||
| Keywords | ||
| the efficiency of the enzyme glucose Oazomez product from the local isolation of the bacterium Streptomyces sp; in converting glucose to fructose | ||
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