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Assessment of the Immunogenic Potential of the Infectious Bursal Disease Vaccine Virus (G-61) in Broiler Chicks | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 6, Issue 4, 2010, Pages 298-307 | ||
| Authors | ||
| ; I.H. Al-Ibady | ||
| Abstract | ||
| Using of numerous methods of vaccination of broiler chicks leading to variation in the average of immune response that elicited against Gumboro vaccine viruses. The study aimed to determine the effect of the immunogenicity of the live attenuated infectious bursal disease (IBD) vaccine virus (G-61 strain) in Broiler Chicks. A total of (160) chicks were divided into four equal groups namely A, B, C and D (40 chicks per group). The chicks of groups(A, B and C) were vaccinated with live attenuated IBD(G-61) vaccine at (14) days old, via the aerosol, intranasal and drinking water routes respectively while chicks of group D were left without vaccination as a control. An indirect enzyme linked immunosorbent assay (ELISA) was used to detect the antibodies titres against infectious bursal disease (IBD) vaccine virus in sera of chicks at (28) days of age. At (29) day of age, chicks of all groups were challenged by using of a virulent IBDV. At (40) day of age, the levels of antibodies against infectious bursal disease virus have been measured by indirect ELISA. The results obtained showed that higher levels of antibodies were noted when the vaccine administered via aerosol route as compared to the intranasal and drinking water. Following challenge of vaccinated chicks, the protection rates noted are correlated to the levels of antibodies elicited. It is concluded that the choice of approach to achieve higher and protective immune status against infectious bursal disease viruses in chicks is to apply IBDV (G-61) vaccine strain in broiler chicks via the aerosol route. | ||
| Keywords | ||
| IBDV; antibody titre; aersol; vaccination methods | ||
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