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THE PROLIFERATIVE PROFILE OF THE RHOMBENCEPHALICDEMILUNE IN THE DEVELOPING RAT CEREBELLUM: A QUANTITATIVE HISTOCHEMICALSTUDY | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 9, Issue 4, 2011, Pages 312-316 | ||
| Authors | ||
| Muthanna AA Al-Kaabi; Huda R Kareem; Ali HA Al-Anbaki | ||
| Abstract | ||
| Background Studies have shown that the cerebellum develops within the dorsal metencephalon creating a rhombencephalicdemilune (RD) which represents the formation site of the cerebellum granular cells progenitors. These studies used different histological techniques but all have provided qualitative information regarding the biosynthesis and cell mitosis at the RD. Objective Quantifying the proliferative activity of the cellsat the RD during the embryonic period. Methods Six age groups from day 16 to day 21 albino rat embryos Rattusrattusnorvegicus were investigated with Ag-NOR staining technique to quantify cell proliferation. Results There wasa statistically significant difference (p<0.01) between cellular activity at different age groups with a surge during embryonic day 18. Conclusions Correlation with other studies revealed that Ag-NOR staining technique, which reflects protein biosynthesis and nuclear mitotic activity, provided a valuable quantitative measure of cellular proliferation in the developing rat cerebellum. Keywords Rhombencephalicdemilune, Rat, Developing Cerebellum, Ag-NOR, Quantitative, Histochemistry | ||
| Keywords | ||
| Rhombencephalicdemilune; Rat; Developing Cerebellum; NOR; Quantitative; Histochemistry | ||
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