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THE USE OF LIPOPOLYSACCHARID AND GLUCAN AS IMMUNMODULATOR OF WOUND INFECTIONS | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 51, Issue 1, 2010, Pages 79-91 | ||
| Authors | ||
| Alice Krikor Melconian; Suhad Saad Mahmood | ||
| Abstract | ||
| One hundred clinical samples were collected from infected wounds of patients admitted in Al-Yarmouk and Al-Kadymia hospital from 1-3 to1-9-2004. The results of aerobic culture appeard that 86% of bacterial isolates showed positive results and the bacteria Pseudomonasaeruginosa and klebsiella pneumonia was the most common isolates. Antibiotic susceptibility test was performed for P.aeruginosa and K.pneumonia and the results appear the two types of bacteria sensitive to imipenium and resistant to amoxiclave and cephalexin. Lps was extracted from the most drug resistant isolate P.aeruginosaa(Pa8) by hot phenol method and partial purified by gel filtration chromatography using sepharos-6-B and the results showed that carbohydrate and protein ratio in partial purified extracted were 37.5%and1.5% respectively,and the extract was free of amino acid. The results of immune by lps in 50Mg and glucan in 10 Mg and use the two substances together in the same concentrations gave best results appear through wound healing of skin that infect by P.aeruginosaa (Pa8) | ||
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