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The validity of Kala-azar rapid detection test in the laboratory diagnosis of visceral leishmaniasis in Baquba | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 7, Issue 4, 2011, Pages 47-53 | ||
| Authors | ||
| Abdul-Razak SH. Hasan; Zainab H. Al-Azawi; Ammar Riyadh Qasem | ||
| Abstract | ||
| Background: visceral leishmaniasis is the most severe form of leishmaniasis. The disease caused by protozoan parasites of the Leishmania genus. It is the second-largest parasitic killer in the world, being responsible for an estimated 500,000 cases each year worldwide. Objectives: To explore the validity of rapid detection test for the laboratory diagnosis of visceral leishmaniasis against the conventional blood smear technique. Patients, materials and methods: The present study was carried out at Al-Batoul Teaching Hospital during the period from January to July 2009. A total of 75 patients with VL were included. 28 (37.3%) of them were female and 47(62.7%) were male. The age range was 1 month- 8 years. Additionally, 30 apparently healthy individuals were enrolled as control groups. 13(43.3%) of them were female and 17(56.7%) were male. Venous blood sample were collected from each subject; sera were separated and kept frozen till use. All sera were tested for anti-rK39 IgG antibodies using the InBios Kala-azar detect rapid test (Seattle, WA) according to the manufacturer's protocol. Results: The results showed that out of 75 patients, 10 (13.3%) and 9 (12%) were positive for kala-azar by microscopical blood film examination and kala-azar rapid screening test respectively. The distribution of positive cases according to the gender revaled hat the infection rate among males was higher than in females, and the most affected age was 1-4 years. Conclusion: The validity of kala-azar rapid setection test was comparable to that of microscxopical examination for detection of kala-azar cases among clinically suspected patients. | ||
| Keywords | ||
| KEYWORDS; kala azar; Black feve | ||
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