Yelderim Bakir, T., Mustafa Qader, A. (2011). Molecular Identification of Giardia duodenalis Parasite Isolates from Human by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism Technique (PCR-RFLP) in Baghdad Province. , 7(4), 54-66.
Tural Yelderim Bakir; Abdullah Mustafa Qader. "Molecular Identification of Giardia duodenalis Parasite Isolates from Human by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism Technique (PCR-RFLP) in Baghdad Province". , 7, 4, 2011, 54-66.
Yelderim Bakir, T., Mustafa Qader, A. (2011). 'Molecular Identification of Giardia duodenalis Parasite Isolates from Human by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism Technique (PCR-RFLP) in Baghdad Province', , 7(4), pp. 54-66.
Yelderim Bakir, T., Mustafa Qader, A. Molecular Identification of Giardia duodenalis Parasite Isolates from Human by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism Technique (PCR-RFLP) in Baghdad Province. , 2011; 7(4): 54-66.

Molecular Identification of Giardia duodenalis Parasite Isolates from Human by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism Technique (PCR-RFLP) in Baghdad Province

Diyala Journal For Pure Science
Article 1, Volume 7, Issue 4, 2011, Pages 54-66
Authors
Tural Yelderim Bakir; Abdullah Mustafa Qader
Abstract
The present study was designed to determine the genotypes of Giardia duodenalis isolates
from human by using polymerase chain reaction – restriction fragment length polymorphism
technique (PCR-RFLP) technique, by amplification of glutamate dehydrogenase (gdh) gene to
provide information about the genetic diversity and their epidemiological and clinical
characteristics in Baghdad province. Thirty isolates from children (1-12 years old) were
processed for (PCR-RFLP). Data corresponding to demographic, social and environmental
variables and presence or absence of symptoms were collected. The glutamate dehydrogenase
(gdh) gene was amplified by using specific primers (GDHiF and GDHiR), the PCR
amplification was observed in 17/30 (56.6%) of the fecal samples, among these 5/17 (29.4%)
samples belonged to genotype A and 12/17 (70.5%) samples belonged to genotype B. Genetic
subgenotypes identified from human fecal samples revealed that, 5/17 (29.4%) were
subgenotype AΙΙ, 9/17 (52.9%) subgenotype BΙΙΙ, and 3/17 (17.6%) subgenotype BΙV.
Genotype B was detected in children with severe symptomatic giardiasis and many of them
had diarrhea while, subgenotype AΙΙ was detected in children with mild symptomatic
giardiasis and without diarrhoea.
Keywords
Key Words; PCR; RFLP; Molecular identification
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