- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Characterization of B-lactamase from local isolate of Staphylococcus aureus | ||
| AL-TAQANI | ||
| Article 1, Volume 23, Issue 3, 2010, Pages 132-138 | ||
| Authors | ||
| Awatif H. Issa; Eiman A. Saeed; Rafid M. Karim | ||
| Abstract | ||
| The Characterization of β-lactamase that isolated from local isolate of Staphylococcus aureus has been studied, the suitable temperature for enzyme activity ranged between (25 to 45)°C with an optimal temperature at (25, 30, 35)°C. The enzyme was retained 83% of its activity after incubation for 10 min. at 45°C, while it has been lost 48% of its activity after 60 min. of incubation. Also, the enzyme was active at acid reactions between (4.5 to 7.5) with an optimal pH (6, 6.5, 7). The enzyme activity was not inhibited by AgNO3, CaCl2, KCl, NaCl, and EDTA, while 2-mercaptoethanol (1mM) inhibited 98% of the enzyme activity and (30µg/ml) of Augmentin inhibited 73% of enzyme activity | ||
|
Statistics Article View: 168 PDF Download: 82 |
||