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Using PCR Technician in Prenatal Diagnosis of Fetal Gender | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 4, Issue 2, 2010, Pages 84-90 | ||
| Author | ||
| Abdulrahman A. Oleiwi | ||
| Abstract | ||
| whole blood samples were obtained from 30 pregnant women at 15 –24 weeks of gestation. DNA was extracted from each plasma or serum sample. To detect the Y-chromosome specific marker DYS14 in the maternal blood, (Polymerase Chain Reaction) PCR were carried out for each DNA extract. The PCR products were analyzed by 1.5% agarose gel electrophoresis and ethidium bromide staining. The results compared with fetal gender after delivery. The result of delivery revealed that 13 pregnant women had a male fetus and the remaining 17 pregnant women had a female fetus and DYS14 was detected in all plasma and serum samples obtained from pregnant women and revealed that 13 pregnant women had a male fetus and the remaining 17 pregnant women had a female fetus. The PCR sensitivity for detecting the gender of fetus from maternal whole blood at 15–24 weeks of gestation was 100% in both plasma and serum, DYS14 was not detected in the DNA from any of the 17 pregnant women carrying a female fetus. The results showed that PCR analysis of maternal plasma and serum can be used to diagnose fetal gender. | ||
| Keywords | ||
| Prenatal diagnosis; maternal blood; fetal gender; PCR | ||
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