- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
The Purification of Maize Dwarf Mosaic Virus (MDMV) and Preparation of its Antiserum | ||
| Rafidain Journal of Science | ||
| Article 4, Volume 22, Issue 4, 2011, Pages 40-48 PDF (0 K) | ||
| DOI: 10.33899/rjs.2011.31298 | ||
| Authors | ||
| Fadl Y. Abbo; Nabeel A. Kassim | ||
| Abstract | ||
| Maize dwarf mosaic virus (MDMV) was diagnosed on corn crop in Ninewah province by using tissue blot immunoassay (TBIA). The virus isolate was propagated in corn plants. The virus was purified by differential centrifugation and precipitated by polyethylene glycol (PEG) and then by sucrose density gradient. The efficiency of purification method was evaluated biologically and spectrometrically, and virus purity was 1.12 . Its antiserum was prepared by using rabbit, the end the dilution point ( titer ) of antiserum was 1/ 512. The efficiency of antiserum was tested by agglutination, and agar double diffusion tests . | ||
| Keywords | ||
| Maize dwarf mosaic virus; Virus antiserum | ||
|
Statistics Article View: 407 PDF Download: 268 |
||