| The present study entalid collection of (50) ear swabs from children patients suffering from Acute suppurative Otitis Media (ASOM) carried at the E.N.T section of the teaching hospital in AL-Diwaniya a City. Whose ages ranged between (1 day- 5 years) during the Period from October 2006 until Jun 2007. The aim of these study is to isolate dominant pathogenic causes and responsible of (ASOM) in Children and effect of some antibiotics on this causes.Staphylococcus aureus bacteria was the dominant cause of Acute suppurative otitis media with the percentage of 49. 23%. Followed by Candida albicans with the percentage of 30.76%, Proteus vulgaris 6.15%, Pseudomonas aeruginosa 4.61 B-haemolytic streptococci, Klebsiella Pneumoniae Haemophilous influeenzae, Staphylococcus epidermidis, Enterobacter clocase, Moraxella catarrhalis with percentage 1.53% Results showed that 80% of the infection cases were caused by one pathogenic factor and 20% of them but more than one factor. The sensitivity test showed that staph. aureus bacteria had the highest Sensitivity to vancomycin 96.87%, Amikacin 78.12%, Tobromycin 68.74% and ciprofloxacin 65%, While showed lowest sensitivity to Lincomycin 3.12% while Proyeus vulgaris showed highest sensitivity to vancomycin 100%, Trobromycin 75%, Amikacin and ciprofloxacin with other antibiotic 50% and showed lowest sensitivity to Tetracyclin with other antibiotics 25%. Pseudomonas aeruginosa bacteria showed highest sensitivity to vancomycin and Amikacin 100%. Tetracyclin and ciprofloxacin with other antibiotics 66.66% while showed lowest sensitivity to Tobramycin and other antibiotics 33.33%. the results of synergistic antibiotics effect on Staph aureus showed the effect between Amikacin with (Tobramycin, ciprofloxacin and tetracycline) had a parentage of 90.47% whilethe synergistic, effect between Tobromycin and (Amikacin , ciprofloxacin and Tetracycline) hada percentage of 42.85%.While synergistic effect on Proteus vulgaris showed the effect between Amikacin with (Tobromycin, ciprofloxacin and Tetracycline) had percentage of 50%. While synergistic effect between Tobromycin and (Amikacin, ciprofloxacin and Tetracycline) had a percentage of 66.66%. The results showed that Candida albicans isolates had the highest sensitivity to Nystatin with inhibition zone rate (10, 11.8, 18.76) mm, Miconazole (0, 8, 15.3) mm, ketoconazole (10, 9.6,14.94) mm, Fluconazole (0, 9.6, 12.6) mm, Griseofulvin (0, 0, 6) mm. |