PLASMID CURING OF LOCAL ISOLATES OF KLEBSIELLA PNEUMONIAE ISOLATED FROM URINARY TRACT INFECTIONS AND ITS ROLE IN MULTIDRUG RESISTANCE

Kassim Ghanima, Kais; Nazar Faraj, Dimah

	PLASMID CURING OF LOCAL ISOLATES OF KLEBSIELLA PNEUMONIAE ISOLATED FROM URINARY TRACT INFECTIONS AND ITS ROLE IN MULTIDRUG RESISTANCE
Iraqi Journal of Science
Article 1, Volume 51, Issue 3, 2010, Pages 415-421
Authors
Kais Kassim Ghanima; Dimah Nazar Faraj
Abstract
Curing of plasmid for 7 local isolates of K. pneumoniae have multi-drug resistance and isolated from urinary tract infections (UTI) by using acridin orange and sodium dodecyl sulphate (SDS) were investigated. These results showed that the local isolates of K. pneumoniae were very resist (100%) to Ampicillin, Pepracillin, Amoxicillin, Penicillin G, Tetracyclin, Erythromycin and Gentamycin, high resist (71–80%) to Cephalexin, Cephalothin, Ceftazidine, Ciprofloxacin, Streptomycin and Tobramycin, moderate resist (43–57%) to Cefaclor, Cephradin and Naldixic acid and weak resist (29%) to Cefotaxime while they were susceptible to Chloramphenicol and Amikacin. t was found that SDS is more efficient in curing than Acridin orange, hence the losing of the resistance of antibiotics by using SDS was in the range (70–100%) while by using Acridin orange was in the range (28– 100%). After curing of plasmid the Antibiotics resistance losed for Pipracillin, Amoxicillin, Penicillin G, Cephalexin, Cephalothin, Ceftazidin, Tetracyclin, Gentamycin and Tobramycin, this result confirm that the resistance of these antibiotics is born on plasmid, while the resistance of Ampicillin, Ciprofloxacin, Erythromycin and Streptomycin not losed, this result confirm that there resistance genes are born on the chromosome.

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