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Propagation of Tamarind (Tamarindus indica L.) Plant by Tissues Culture | ||
| Rafidain Journal of Science | ||
| Article 6, Volume 22, Issue 4, 2011, Pages 68-78 PDF (0 K) | ||
| DOI: 10.33899/rjs.2011.31505 | ||
| Author | ||
| Sajida A. Abood | ||
| Abstract | ||
| The role of some growth regulators in the initiation and callus growth from seedling's stem explants of tamarind (Tamarindus indica L.) was carried out.Benzyladenine (BA) was used at concentrations (0.0, 0.5, 1.0, 1.5, 2.0) mg/l, while Naphthaleneacetic acid (NAA), Dichlorophenoxyacetic acid (2,4-D) and Indolebutyric acid (IBA) for each were used at various concentrations (0.0, 0.5, 1.0, 1.5, 2.0) mg/l. The result indicated that the best medium for growth callus was the MS medium containing 1.0 mg/l BA, depending on callus fresh weight after 90days of growth. The addition of 3% of sucrose to MS medium stimulated the initiation of callus well. Moreover, shoot development occurred on callus grown on medium containing BA (1.5mg/l) after 70 days of growth. The differentiated callus was subcultured on hormone-free medium and then the plants developed can be readily transferred to the soil after harding | ||
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