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Qualitative and Quantitive Analysis of Amino Acids Isolated from Protein of Phoenix dactylifera and Determination of Molecular Weight by Gel Filtration (part I) | ||
| Rafidain Journal of Science | ||
| Article 9, Volume 22, Issue 4, 2011, Pages 111-128 PDF (0 K) | ||
| DOI: 10.33899/rjs.2011.31535 | ||
| Author | ||
| Dina I. M. Al-Sawaf | ||
| Abstract | ||
| The research was concerned with Amino Acid Analysis of the protein isolated from the crude aqueous extract of ground date seeds Phoenix dactylifera. Two types of analysis acidic and basic hydrolysis were performed for (72 hours). Acidic hydrolysis showed absence of some amino acids and a decrease in the intensity of the peaks. On the other hand, basic hydrolysis showed higher intensity of peaks for the same aqueous extract. The research also involved separation of the proteinous components from the aqueous extract of ground date seeds Phoenix dactylifera. Three major proteins (a, b, c) were isolated and their molecular weight were determined by gel filtration and showed the following values (>2000000, 45000, 251) Dalton Respectively | ||
| Keywords | ||
| Phoenix dactylifera; Amino acid analysis; gel; filtration technique | ||
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