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EXTRACTION AND PURIFICATION OF β-galactosidase FROM NEW BORN SHEEP BRAIN. | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 51, Issue 3, 2010, Pages 437-443 | ||
| Author | ||
| Ali Noori Abed | ||
| Abstract | ||
| β -galactosidase enzyme was extracted from the new born sheep brain by nine solutions, It was found that the0.2 M sodium acetate and 0.2M NaCl give a highest specific activity of crude enzyme. This enzyme was concentrated by four methods, includes: concentration by ammonium sulfate (20-60%); old acetone; ethanol (30%-60%) and by ultra filtration. It was found that the cold acetone was the better. The purification folds were about 124.78 times and yield about 77.39%. The molecular weight of this enzyme about 185.942 Kilo Dalton. | ||
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