Effect of crude and steroid free bovine follicular fluid on some hematological and biochemical parameters and ovarian development in rats

Abdulkadem AL-Zamely, Hayder; Abdulazeez AL-llozy, Mohammad

	Effect of crude and steroid free bovine follicular fluid on some hematological and biochemical parameters and ovarian development in rats
Kufa Journal For Veterinary Medical Sciences
Article 1, Volume 2, Issue 1, 2011, Pages 93-105
Authors
Hayder Abdulkadem AL-Zamely; Mohammad Abdulazeez AL-llozy
Abstract
Abstract: Ovarian growth and function is under the control of pituitary gonadotropins and various local intraovarian factors. The present study has been undertaken to assess the efficacy of crude follicular fluid and steroid free FF taken from bovine ovaries on some hematological aspect as well as ovarian growth and function of mature female Wistar rats. Follicular fluid aspirated from large size Graffian follicles of abattoir – derived bovine ovaries was made cell free by centrifugation and steroid free by charcoal treatment. follicular fluid ‎ has been fractioned using ammonium sulphate precipitation at various saturation levels and then subjected to get filtration chromatography. The 20 – 40% fraction yielded the detectable peak and the average yield of peptide was 0.659 mg/ml of FF . The crude and steroid free FF were tested for effects on some hematological parameters and ovarian and uteri weights, numbers of different types of ovarian follicles and assessment of histophysiological appearance. Twenty four mature female Wistar rats were randomly assigned into three equal groups (control and two treatment groups), injected with normal saline (Control), SFFF (T1) and CFF (T2) for 4 consecutive days. At the fifth day, all female rats were anesthetized, dissected and blood samples were obtained for hematological study and ovaries and uteri were removed , weighted and fixed in formalin 10% for histophysiological study . The results revealed no significant effects in Hb concentration , PCV , AST , concentrations, cholesterol , urea , and glucose concentrations . On the other hand , the result revealed no effect on uteri and ovarian weights , an increment in the numbers of primary and secondary follicles and a decrement in the number of Graffian follicles . Within each group , the results showed that primary follicles recorded the highest number among the different types of ovarian follicles. While the highest total number of the different types of ovarian follicles were recorded in CFF and SFFF groups compared with control . The present study demonstrated no side effect on blood constituents as well as the effect of a bovine intrafollicular factor/s regulating ovarian growth and function in rats , especially the development of primary and secondary follicles unlike Graffian follicles which underwent from the inhibition .

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