- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
EFFECT OF SOME CHEMICAL AND PHYSICAL FACTORS ON THE COCCI SHAPE FORMATION OF HELICOBACTER PYLORI ISOLATED FROM PATIENTS WITH DUODENUM ULCER | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 52, Issue 3, 2011, Pages 292-299 | ||
| Authors | ||
| Dhuha Saad Saleh; Hala Mouyed Radif | ||
| Abstract | ||
| Helicobacter pylori was islotaed localy from patients with Duodenal Ulcer. Two biopsies were taken from each patient, the first used for isolation of H. pylori using ski row medium, the second used for rapid urease test. Optimum growth temperature was 37 cْ. Isolated H. pylori was identified according to bacterial shape, graw stain, biochemical test, sensitivity to cephalothin & resistance to nalidixic acid. The result showed that component of culture media, light, & oxygen are important factors affecting Hansformed from spiral to coccoid shapes. It has been noticed that using Brain – heart infusion a gar and blood a gar base with growth supplement give &5% blood, percentage transformation to coccoid shape was less than using the same culture media without blood and growth supplements. Results indicated that avoiding exposure of culture media to light and oxygen (more than 5%) maintain spiral shape of H. pylori for 20 – 30 days when stored at 4 cْ taking in consideration a pH of (6.9 – 8). | ||
|
Statistics Article View: 73 PDF Download: 56 |
||