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Isolation Of Proteus vulgaris urease Producer From Children Infected with Urinary Tract Infections | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 6, Issue 2, 2010, Pages 260-274 | ||
| Author | ||
| Hadi Rahman Rasheed AL-Taai | ||
| Abstract | ||
| 709 urine sample were collected from children suffering urinary tract infection through period March - September 2007. The results indicated that(514 ) 72.4% of the samples were Positive for bacteriological culture, and( 87)16.9 %of isolates were Proteus .The distribution infection was 70.11% female and 29.89% male ,and18.4% of isolates were Proteus vulgaris. All isolates were urease producer, and specific activity 1.74 unit/mg protein for active isolates. Different concentration of selected heavy metals were used to assess tolerance of Proteus vulgaris to their toxic ions, results indicated that (33.33, 25, 50, 16.16, 16.16, 25 )% of isolates were tolerant ( 0.1, 1.5, 2, 3, 0.03, 1.5 ) mM from Hg , Cd , Zn ,Cu , Cr ,Co ) respectively . The sensitively of these isolates were tested against (13) antibiotics. The results showed that antibiotic Ampicillin was non effective with percentage of resistance( 100%) , while the antibiotic Ciprofloxacin was found to be the most effective against local isolates with a percentage of resistances 0% . The M.I.C was determined for Ten antibiotics, results showed differences values of M.I.C among isolates, some was able to resist (128-1024) µg/ ml β-lactam antibiotics, while their resist (16-512) µg / ml cephalosporin antibiotics. Results showed linkage between antibiotics and heavy metals with percentage 91.66 % Ampicillin, Tetracycline and Mercury. | ||
| Keywords | ||
| Key Words; urease; antibiotic; Heavy Metals | ||
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