DETECTION OF B1 GENE OF TOXOPLASMA GONDII IN BLOOD OF PREGNANT AND ABORTIVE WOMEN INFECTED WITH THIS PARASITE

Hori Zghair, Khawla; Hadi Hassani, Hayfa; Haiawi Mahmood, Souhaila

	DETECTION OF B1 GENE OF TOXOPLASMA GONDII IN BLOOD OF PREGNANT AND ABORTIVE WOMEN INFECTED WITH THIS PARASITE
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 8, Issue 3, 2010, Pages 42-48
Authors
Khawla Hori Zghair; Hayfa Hadi Hassani; Souhaila Haiawi Mahmood
Abstract
Background: Primary maternal infection with toxoplasmosis during gestation and its transmission to the fetus continue to be the cause of tragic yet preventable disease in offspring. Objective: This study was aimed to investigate the utility of nested PCR (nPCR) technique for detection recent infection with Toxoplasma gondii in blood of pregnant and abortive women. Methods: One hundred twenty women were included in this study with a history of single or repeated abortion and thirty women with normal pregnancy were used as a control. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by an enzyme-linked immunosorbent assay (ELISA) and detection of B1 gene of T. gondii by nPCR. Results: The results indicated that 43.33% of abortive women were exposed positive for anti-Toxoplasma antibodies, 4.16% of them had IgM, 25.83% had IgG, and 13.33% had both IgM and IgG, and 56.55% had no antibodies. Subsequently, nested PCR analysis was used to detecting T. gondii DNA in blood of abortive women. It was found that 15.83%of abortive women exposed positive result for B1 gene of T. gondii, those abortive women involved 10.52% of them with IgM, 31.57%with IgG, and 26.31% with both IgM and IgG, and 31.57% of them had none anti-Toxoplasma antibodies. Conclusion: It can be concluded that nPCR assay in blood has advantage in detection of recent and active toxoplasmosis. Key Words: Toxoplasma gondii, nested PCR, toxoplasmosis.
Keywords
Toxoplasma gondii; Nested PCR; Toxoplasmosis

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