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Detection of some Alkaloids and Fatty Acids to Plant and Callus of Purslane (Portulaca oleracea L . ) | ||
| Rafidain Journal of Science | ||
| Article 7, Volume 22, Issue 2, 2011, Pages 77-99 PDF (0 K) | ||
| DOI: 10.33899/rjs.2011.32381 | ||
| Author | ||
| Mira A. Al – Katib* | ||
| Department of Biology College of Education Mosul University | ||
| Abstract | ||
| The study results showed that the best supporter media for callus induction from local purslane (Portulaca oleracea L.) stems and leaves sterile explants , those MS media(Murashige and Skoog medium) supporting with hormone regulators BA(Benzyl adenine), NAA(Naphthalene Acetic Acid) in concentrations 1.0 , 2.0 , 4.0 , mg/L from each of them as well as MS medium containing BA : NAA in 2:1 mg/L respectively , and the callus was inculcation during 15-20 day . The result of separating alkaloids from callus of stems and leaves and shoots of plant showed in Infrared Spectrophotometer technique (IR) separating of Oleracein B and Oleracein D that degree in purity and the best of it appear in stems callus extract . when Thin Layer Chromatography Technique (TLC) was made for extracted alkaloids , Rate of Flow (Rf) values appeared very comparatively to these samples (0.77, 0.82, 0.80) to each of stems callus alkaloid, leaves callus alkaloid and plant alkaloid respectively , then retention time record for it , and main band recognized in plant alkaloid at 4.755 min , also 4.646 min and 4.657 min in stems callus alkaloid and leaves callus alkaloid respectively by using High-Performance Liquid Chromatography (HPLC) technique , that result prove of Oleraceins (B, D) occurrences in samples of purslane . The fatty acids which occur in purslane plant , calli of stems and leaves are detect by using Capillary Gas Chromategraph (CGC) technique , the plant extract showed 12 fatty acids in it , but in low concentration compared with that appear in leaves callus extract for most fatty acids especially omega – 3 fatty acid (C18: 3) , when the stems callus extract record the lower ratio of fatty acid occurrences that it was 3 fatty acids only (C16: 0 , C16: 1, C18: 1). | ||
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