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In vitro propagation of some tomato (Lycopersicon esculentum Mill.) hybrids | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 1, Issue 1, 2007, Pages 52-60 | ||
| Authors | ||
| Kadhum Muhammed Ibrahem; Hamza Musa Kadhum; Ali Abadi Manea | ||
| Abstract | ||
| Seeds of four tomato hybrids Ginan, Nirtia, Speedy and Rola were cultured on aseptic germination medium after surface sterilization with NaOCl. Shoot tips 1cm length were dissected and grown on MS medium supplemented with Benzyl adenine (BA) and Indole acetic acid (IAA) for multiplication. Callus was induced on hypocotyls cultured on MS medium supplemented with BA under light intensity of 1000 lux and 25 0C. Results showed that NaOCl at 4% completely disinfected seeds and resulted in pathogen free seedlings. Results also showed that a concentration of 4 mg/l of BA was superior in increasing callus fresh and dry weights. Shoot multiplication was achieved on the same medium supplemented with 2 and 0.8 mg/l of BA and IAA respectively. | ||
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