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The effect of gamma radiation on callus growth and plantlet regeneration of four tomato hybrid Lycopersicon esculentum mill In vitro | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 3, Issue 1, 2009, Pages 56-62 | ||
| Author | ||
| Rana A.H.Al-roomi | ||
| Abstract | ||
| Calluses were induced from four tomato hybrid (Davista, superDavista, Muntcarloo, Hymar). On MS medium supplemented with 2, 4-D Auxin at concentration of 4 mg/L. The Callus were divided into two groups the first one was affected with gamma radiation at the doses (0,2,4,6,8)Gray and re cultured on the same mentioned medium. The second group was also affected with gamma radiation with the same doses mentioned above and re- cultured on MS media supplemented with 0.5 mg/L Kinetin for regeneration. Fresh and dry weight was measured after 8 weeks. The number and length of shoots were measured as indicators after 6 weeks. Results revealed that the dose 2 Gray was superior to give highest rate of fresh and dry weight reaching 238.5 and 43.1 mg respectively and the hybrid super Davista was superior in giving the highest fresh and dry weight reaching 231.6 and 30.2 mg respectively. Moreover the results revealed that control treatment was superior in giving the highest rate in number and length of plantlets. The 2 Gray treatment showed a significant effect on the rate and length of plantlets. In addition, there were significant differences among the cultivars in most characters mentioned above. | ||
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