- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Diisttrriibuttiion off allgD,, llasB,, piillB and nan1 genes among MDR clliiniicall iisollattes off Pseudomonas aerrugiinosa iin rrespectt tto siitte off iinffecttiion | ||
| Medical Journal of Tikrit | ||
| Article 1, Volume 2, Issue 172, 2016, Pages 148-160 | ||
| Authors | ||
| ; ad Mahmood Ra | ||
| Abstract | ||
| A number of Pseudomonas aeruginosa isolates collected in a previous study from patients admitted to Diyala hospital in Diyala city /Iraq, were re-identified and tested for their susceptibility to fourteen antibiotics. The results showed that all isolates 20(100%) were resistant to ampicillin, augmentin, cephalexin and carbenicillin , followed by cefotaxime (95%) ,ceftriaxone (90%) ,gentamicin (75%), ceftazidime (70%), pipracillin (65%), tobramycin (50%), ciprofloxacin (45%), amikacin (45%), then norfloxacin (30%), while all isolates 20 (100%) were sensitive to ofloxacin. Twenty multidrug resistant (MDR) isolates (five isolates from each infection source)which had the ability to resist must of tested antibiotics were chosen to study their abilities to produce five virulence factors according to the standard methods described by Cruickshank et al., 1975 and the results showed that all isolates 20(100%) had the ability to produce hemolysin and lecithinase, followed by alkaline proteases 12(60%), adhesion factors 19(95%) then urease 9 (45%). Then, these isolates were tested to evaluate the prevalence of four virulence genes (gene encoding for alginate (algD), elastase (lasB), type IV fimbrial biogenesis protein PilB (pilB) , and neuraminidase (nan1) ) by PCR technique using specific primers. The results showed that algD and lasB genes were widely disseminated (100%) in all studied isolates with no difference according to site of infection( source of isolates) , while pilB and nan1 genes were low disseminated (35%) and (15%),respectively, in these isolates with obvious distribution of pilB gene in respect to site of infection, since the results showed that this gene was widely disseminated in Pseudomonas aeruginosa isolated from wound infections 3/5 (60%), followed by 2/5 (40%) of ear isolates , then 1/5 (20%) for each of urine and burn isolates. For nan1 gene the results showed that 2/5 (40%) of urine isolates possess this gene ,followed by 1/5 (20%) of ear isolates ,while absolute absence of this gene was recorded in wound and burn isolates. From the results of PCR, we can concluded that algD and lasB genes may be important in all types of infections, while the prevalence of pilB and nan1 genes may be related to infection site (source of isolates). Our results on nan1 gene dissemination may support the hypothesis of Mitov et al., that the molecular-genetic detection of nan1 gene may be used as an indirect measure of CF pulmonary disease evolution because of the high prevalence of this gene in strains from CF infections (which recorded by many researchers) and its low dissemination in isolates from infections rather than CF (as we reported) . | ||
| Keywords | ||
| Diisttrriibuttiion off allgD; llasB; piillB and nan; Diisttrriibuttiion; piillB | ||
|
Statistics Article View: 114 PDF Download: 21 |
||