DETECTION OF IL-10, IFN-γ AND IL-8 IN SERA OF PATIENTS WITH RECURRENT SPONTANEOUS ABORTION

Hussain, Amal; Abdul Mohymen, Nidhal

	DETECTION OF IL-10, IFN-γ AND IL-8 IN SERA OF PATIENTS WITH RECURRENT SPONTANEOUS ABORTION
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 6, Issue 2, 2008, Pages 58-65
Authors
Amal Hussain; Nidhal Abdul Mohymen
Abstract
Background: Th1-type cytokines secretion such as IFN-γ, and Th2 cytokines such as IL-10, have been shown to exert deleterious effects on pregnancy, inhibiting fetal growth and development Objective: Estimation of Interleukin-10 (IL-10), IL-8 and IFN-γ levels in sera of patients with recurrent spontaneous abortion (RSA) using ELISA method. Method: A total of one hundred and nineteen women, ranged from the mean age (23.9 – 28.5)years, were enrolled in the current study and were further classified into three categories: Group A- Recurrent spontaneous abortion (RSA): n= 62 women, with a mean age of (28.5 + 0.68);Group B- non- recurrent spontaneous abortion (non-RSA): n= 34 women, with a mean age of (26.4 ± 0.85)and group C- Control (successful pregnancy): n= 23 women, with a mean age of (23.9 ± 0.88). From each patient and control blood sample was collected and serum was eparated. Estimation of Interleukin-10 (IL-10), IL-8 IFN-γ levels in sera of patients was done using ELISA method. Result: the current study failed to demonstrate a significant difference in circulating levels of IL-8 between RSA and control group (p> 0.05) and no significant different between non-RSA and control (p>0.05) . IFN-γ expression is significantly increased (p<0.001) in women with RSA and non-RSA compared with successful pregnancy. Defective IL-10 expression in women with RSA and non-RSA .The ratio of IFN-γ: IL-10 was found to be highly significant (p<0.001) in aborted women. IL-8 was expressed in high levels in aborted women (RSA and non-RSA) and those with successful pregnancy, but no significant difference (p>0.05) was found when compared between successful pregnancy and RSA or non-RSA, whereas highly significant difference (p<0.001) was found between RSA and non-RSA. Conclusions: IFN-γ expression is highly significant increased (p<0.001) in women with RSA and non-RSA compared with successful pregnancy, indicating that Thl cytokines might well be implicated in adversely affecting pregnancy. And defective IL-10 expression in women with RSA and non-RSA might be documentary to the previous studies on the possible defect in Th2 cytokines production in these patients.
Keywords
Recurrent spontaneous abortion; Interleukin; IFN; ELISA

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